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pgl3 basic empty vector  (Addgene inc)


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    Structured Review

    Addgene inc pgl3 basic empty vector
    Pgl3 Basic Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+pgl3+basic+plasmid/pGL3+Basic+Control+Vector+(Plasmid+%23137707)/pm40373767-543-11-16
    Average 93 stars, based on 49 article reviews
    pgl3 basic empty vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Luciferase:

    Article Title: Lysine acetyltransferase 14 mediates TGF-β-induced fibrosis in ovarian endometrioma via co-operation with serum response factor
    Article Snippet: The wild-type form of the 3′UTR region of the ACTA2 promoter containing the CArG box was subcloned into the pGL3 reporter vector (#E1751, Promega, RRID: Addgene_48743). .. For the luciferase assay, the pretreated primary human EcESCs were plated on 24-well plates and co-transfected with 0.5 μg of pGL3-α-SMA-pro-Luc or empty (pGL3-Basic) plasmids together with 0.05 mg of pRL Renilla luciferase reporter vector (#E2241, Promega, RRID: Addgene_44379) using Lipofectamine 3000 according to the manufacturer’s instructions. ..

    Article Title: Lysine acetyltransferase 14 mediates TGF-β-induced fibrosis in ovarian endometrioma via co-operation with serum response factor.
    Article Snippet: The wild-type form of the 3′UTR region of the ACTA2 promoter containing the CArG box was subcloned into the pGL3 reporter vector (#E1751, Promega, RRID: Addgene_48743). .. For the luciferase assay, the pretreated primary human EcESCs were plated on 24-well plates and co-transfected with 0.5 μg of pGL3-α-SMA-pro-Luc or empty (pGL3-Basic) plasmids together with 0.05 mg of pRL Renilla luciferase reporter vector (#E2241, Promega, RRID: Addgene_44379) using Lipofectamine 3000 according to the manufacturer’s instructions. ..

    Plasmid Preparation:

    Article Title: PI3K p110α isoform-dependent Rho GTPase Rac1 activation mediates H2S-promoted endothelial cell migration via actin cytoskeleton reorganization.
    Article Snippet: .. Plasmids-pcDNA3-EGFP-Rac1-T17N (Addgene plasmid 12982) as the dominant negative plasmid of human Rac1 gene and pcDNA3-EGFP (Addgene plasmid 13031) as the empty control vector were both obtained from the plasmid repository at Addgene (Cambridge, MA, USA). ..

    Article Title: Hepatic lipid remodeling in cold exposure uncovers direct regulation of bis(monoacylglycero)phosphate lipids by phospholipase A2 group XV.
    Article Snippet: .. These 293T cells were plated in 96-well plates and transfected with pGL3 Basic empty vector, pPparg (addgene#8896), or pGL3 Pla2g15 R1-R5, and pCMV-Renilla control plasmid (addgene#118066). .. After 48h of growth at 37◦C, DualGlo Luciferase Assay System (Promega, E2940) was used to assess luciferase and Renilla activity.

    Article Title: Interference with ENO2 promotes ferroptosis and inhibits glycolysis in clear cell renal cell carcinoma by regulating Hippo‑YAP1 signaling
    Article Snippet: ENO2 small-interfering (si)RNAs, namely si-ENO2#1 and si-ENO2#2, were synthesized by Shanghai GenePharma Co., Ltd., and the corresponding negative control (si-NC) was also obtained. .. The YAP-overexpressing plasmid DNA (OV-YAP) used to overexpress the YAP gene in cells, and an empty vector control, were obtained from Addgene, Inc. ..

    Dominant Negative Mutation:

    Article Title: PI3K p110α isoform-dependent Rho GTPase Rac1 activation mediates H2S-promoted endothelial cell migration via actin cytoskeleton reorganization.
    Article Snippet: .. Plasmids-pcDNA3-EGFP-Rac1-T17N (Addgene plasmid 12982) as the dominant negative plasmid of human Rac1 gene and pcDNA3-EGFP (Addgene plasmid 13031) as the empty control vector were both obtained from the plasmid repository at Addgene (Cambridge, MA, USA). ..

    Control:

    Article Title: PI3K p110α isoform-dependent Rho GTPase Rac1 activation mediates H2S-promoted endothelial cell migration via actin cytoskeleton reorganization.
    Article Snippet: .. Plasmids-pcDNA3-EGFP-Rac1-T17N (Addgene plasmid 12982) as the dominant negative plasmid of human Rac1 gene and pcDNA3-EGFP (Addgene plasmid 13031) as the empty control vector were both obtained from the plasmid repository at Addgene (Cambridge, MA, USA). ..

    Article Title: Hepatic lipid remodeling in cold exposure uncovers direct regulation of bis(monoacylglycero)phosphate lipids by phospholipase A2 group XV.
    Article Snippet: .. These 293T cells were plated in 96-well plates and transfected with pGL3 Basic empty vector, pPparg (addgene#8896), or pGL3 Pla2g15 R1-R5, and pCMV-Renilla control plasmid (addgene#118066). .. After 48h of growth at 37◦C, DualGlo Luciferase Assay System (Promega, E2940) was used to assess luciferase and Renilla activity.

    Article Title: Interference with ENO2 promotes ferroptosis and inhibits glycolysis in clear cell renal cell carcinoma by regulating Hippo‑YAP1 signaling
    Article Snippet: ENO2 small-interfering (si)RNAs, namely si-ENO2#1 and si-ENO2#2, were synthesized by Shanghai GenePharma Co., Ltd., and the corresponding negative control (si-NC) was also obtained. .. The YAP-overexpressing plasmid DNA (OV-YAP) used to overexpress the YAP gene in cells, and an empty vector control, were obtained from Addgene, Inc. ..

    other:


    Transfection:

    Article Title: Hepatic lipid remodeling in cold exposure uncovers direct regulation of bis(monoacylglycero)phosphate lipids by phospholipase A2 group XV.
    Article Snippet: .. These 293T cells were plated in 96-well plates and transfected with pGL3 Basic empty vector, pPparg (addgene#8896), or pGL3 Pla2g15 R1-R5, and pCMV-Renilla control plasmid (addgene#118066). .. After 48h of growth at 37◦C, DualGlo Luciferase Assay System (Promega, E2940) was used to assess luciferase and Renilla activity.



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    Image Search Results


    Results from gene reporter assays and transcriptional activity of the characterized promotor haplotypes.

    Journal: Genes

    Article Title: Functional Analysis of Promoter Variants in Genes Involved in Sex Steroid Action, DNA Repair and Cell Cycle Control

    doi: 10.3390/genes10030186

    Figure Lengend Snippet: Results from gene reporter assays and transcriptional activity of the characterized promotor haplotypes.

    Article Snippet: Similar experiments were performed with the empty promoterless pGL3-Basic plasmid (Promega) and the SV40-driven Firefly Luciferase pGL3-control plasmid (Promega, Madison, WI, USA) as negative and positive controls, respectively.

    Techniques: Activity Assay, Luciferase, Plasmid Preparation, Clone Assay, Control, Expressing